ÈËÆÞÓÕ»ó

Fire in the Belly

Photo by Matt D’Annunzio

Prof. Bob Kaplan and his students probe deep questions about evolution, ecology, and the fire-bellied toad.

By Geoff Koch

In the summer of 1987, Chris Marshall ’89 found himself on a mountainside in South Korea with a big headache. 

Marshall had come to study insect life in the ponds of Tut’a-san Mountain over the misgivings of Prof. Bob Kaplan [biology 1983–]. Given the remote location and long hours in the field, Kaplan had thought the experience was best suited for juniors or seniors, but after much wheedling on Marshall’s part, he had finally relented and told the sophomore he could come. Unfortunately, it turned out that the bugs he wanted were in short supply. Marshall needed a new research project—fast—or his inaugural field experience was going to be an embarrassing flop.

At night Marshall lay awake listening to the sounds of Oriental fire-bellied toads, Bombina orientalis, calling to each other in nearby ponds, and wishing he had brought along a book to read. There was no shortage of frogs (Bombina is actually a frog) by the sounds of things. The next day, Kaplan suggested that Marshall make a virtue of necessity and switch from bugs to frogs—a decision that salvaged Marshall’s summer and taught him the value of improvising in the field. 

He also handed Marshall Siddhartha by Herman Hesse. The slim novel of spiritual self-discovery was important to him, Kaplan said gravely, so much so that he read it again every few years.

“I didn’t get it,” Marshall says today, laughing. “A guy, a journey, blah blah. I was like, You read this TWICE?’”

So I ask Kaplan: How many times has he read Siddhartha in the three decades he’s been at ÈËÆÞÓÕ»ó?

“Oh jeez. No way!” he writes. “At least once.” 

The response seems consistent with a certain fire in the belly that marks his approach to science—an approach that generations of students have come to adore, even though, during Kaplan’s time at ÈËÆÞÓÕ»ó, biology has been turned inside out by new technologies and the ability to probe life at the level of DNA.

Kaplan has adapted—as any evolutionist would—but only to a point. In lectures, lab experiments, and fieldwork, he insists on keeping the focus on whole organisms. It’s not that genetic wizardry isn’t fabulous. Kaplan just happens to think that when it comes to understanding the implications of a changing environment, frogs offer a unique opportunity—and warning.

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KNOT OF TOADS: Mari Cobb ’16, Prof. Kaplan, and Taylor Stinchcomb ’14 contemplate a tank of fire-bellied toads (which are technically frogs—if you need more explanation, ask a bio major).

KNOT OF TOADS: Mari Cobb ’16, Prof. Kaplan, and Taylor Stinchcomb ’14 contemplate a tank of fire-bellied toads (which are technically frogs—if you need more explanation, ask a bio major).
Photo by Matt D'Annunzio

I meet him on a sunny day in October at his office in the Griffin Memorial Biology Building. Before we settle in to talk, he wants to show me the lab next door.

In one room, at a long bench, Mari Cobb ’16 is at a microscope, separating Bombina embryos, which look like miniature tapioca pearls, for an experiment that Kaplan’s intro biology class will run in the ÈËÆÞÓÕ»ó canyon. The source of those embryos is a warm, windowless room filled with tanks of water, where 400 inky spots of movement dart amid the gurgle of water and the hum of pumps.

Kaplan is explaining how each frog was once an embryo assigned to a ÈËÆÞÓÕ»ó student in the intro class. Conveniently, Bombina has a lifecyle that, with a little help from added hormones, dovetails with ÈËÆÞÓÕ»ó’s academic calendar. It’s embryo to frog with a bright yellow-orange belly in just 12–14 weeks.

All students can get the pleasure, familiar to any kid who ever came home lugging a bucket of tadpoles, of watching an astonishing act of metamorphosis. Upper-division students interested in more serious research make use of the Bombina factory too.

Taylor Stinchcomb ’14 is an environmental studies major with an emphasis on biology. For her thesis, supervised by Kaplan, she is exploring how herbicides affect Bombina embryos and larvae. Other scientists have found that herbicides can wreak havoc on a frog’s sexual machinery. Stinchcomb is trying to figure out just how little weed killer it takes to knock the frogs out of the ecosystem.

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Kaplan grew up in Brooklyn. He got interested in biology the same way many of his students do—by spending summers traip-sing around in the woods and looking for critters. In his case, it was eastern red-back salamanders in New York’s Adirondack Park, which has an area larger than Yellowstone, Yosemite, the Grand Canyon, Glacier, and Great Smoky Mountains combined. It was and remains a good place to observe nature, despite the fact that it’s within a day’s drive of 60 million people.

After he’d earned degrees from Brooklyn College and the City University of New York, Kaplan headed west in 1978 to do a postdoc at Berkeley’s Miller Institute for Basic Research in Science and came to ÈËÆÞÓÕ»ó five years later.

Kaplan has always insisted that research go beyond the lab. This fall, as he’s done nearly every year, he took a group of students to Wahkeena Falls, about 30 miles east of Portland in the Columbia Gorge. Headlamps are required on this nighttime hike—local amphibians, like the Pacific giant salamander, are most active after dark.

During his ÈËÆÞÓÕ»ó days, Patrick Phillips ’86 went on one of these trips with Kaplan. Phillips remembers holding jellied clusters of salamander eggs that looked like little translucent jewels—miniature embryos clearly visible when the beam from his headlamp was aimed just so.

“It was breathtaking, just like naked biology, right there in front of you,” he says.  

Today Phillips is a professor of biology and associate vice president for research at University of Oregon. His lab is heavy into the modern tools of biology, including statistical genetics, DNA sequencing, and computer simulations. However, he says the basic questions in biology, the ones immediately evident to those students wearing headlamps, haven’t changed: How does that little jewel develop into a salamander? And how do changes in the environment affect its growth?

Global warming poses a serious threat to amphibians, especially those that breed in ponds, because they can’t regulate their body heat. Many studies have examined the effect of rising average temperature on amphibian development, but virtually none has looked at the impact of thermal variation, where the daily highs and lows of a breeding pond spike erratically due to higher carbon dioxide levels in the atmosphere.

HARDY CREW. Prof. Kaplan in the field in Korea in 2002 with (left to right) Martha Baugh ’01, daughter Jessica, Lindsay Fuchs ’05, and Aaron Clark ’00. In the passenger seat is Choi Geum Sook; driving is her husband, Kim Won Ki. The couple have been doing ground support for Kaplan’s Korean fieldwork since 1985.

HARDY CREW. Prof. Kaplan in the field in Korea in 2002 with (left to right) Martha Baugh ’01, daughter Jessica, Lindsay Fuchs ’05, and Aaron Clark ’00. In the passenger seat is Choi Geum Sook; driving is her husband, Kim Won Ki. The couple have been doing ground support for Kaplan’s Korean fieldwork since 1985.

In a groundbreaking paper published in BMC Ecology in May 2013, Kaplan, Phillips, and several other ÈËÆÞÓÕ»óies—Juliana Arrighi ’07, Advait Jukar ’11, and Ezra Lencer ’06—found that hotter hots and colder colds tend to produce Bombina tadpoles with longer snouts and shorter tails. Sounds like an innocuous result, unless you’re a tadpole. That longer snout and shorter tail would make you a slow, top-heavy, awkward swimmer, and thus easier pickings for predators, which, in a macabre frog-eat-frog twist, often include other amphibians. The takeaway: thermal variation is a critical factor for amphibian survi-val and can’t be predicted by simply plugging higher temperatures into a model.

The experiment is an elegant example of the power of looking at whole organisms. “Parsing genomes isn’t going to stall the huge biodiversity crisis that’s going on right now,” says Elizabeth King ’93, another one of Kaplan’s students and now an assistant professor of ecology at the University of Georgia. “Ultimately there is no human health without functioning ecosystems. The more science about whole organisms and ecosystems that’s done, the more places there are like Bob’s classroom and lab, the better off the planet will be, especially when those students are bright, inventive, out-of-the-box thinkers like ÈËÆÞÓÕ»ó students.”

Kaplan has published 34 papers on evolution and ecology and helped launch the scientific careers of many ÈËÆÞÓÕ»ó alumni. But it is striking how often his former students reach beyond the microscope to describe his impact on their lives. King has fond memories of Kaplan and his wife, Linda, and daughter, Jessica, coming to visit her in Kenya in the mid-1990s after she graduated from ÈËÆÞÓÕ»ó. Kaplan was in Africa exploring whether Hyperolius viridiflavus, the common reed frog, might be suitable for study in his lab back in Portland. Ultimately it didn’t pan out due to onerous export restrictions. She remembers scoping out potential field sites with Kaplan, who at one point considered taking over an abandoned camp used by camel researchers. How to forget Jessica, then in elementary school and now an anthropology doctoral student at University of California, Santa Barbara, practicing her violin while hippos bellowed in the background? Or looking out over a sea of pink flamingoes at Lake Bogoria, where Ernest Hemingway had once camped?

Kaplan has led students on summer trips to Korea for 28 years now. Their pilgrimage sparks considerable local interest—a South Korean TV news magazine broadcast a one-hour show about his work in 2009. While living conditions in his remote Korean research sites have improved since the ’80s, they are still off the grid in every way. Kaplan and the students can now drive all the way to the sites, eschew tents for rustic cabins belonging to his friend, Kim Won Ki, and use the car battery to recharge their laptops. (South Korea may be known around the world for its cell phones, but when Jukar and Kristy Gonyer ’10 went on the trip in 2009, the group had just one phone among them. Getting reception required walking 30 minutes from camp.) 

Jukar is now pursuing environmental science at George Mason University, and Gonyer coordinates ÈËÆÞÓÕ»ó’s science outreach program—started by Kaplan in the mid-1990s. On her Korea trip, she brought Life of Pi, another novel of spiritual discovery. Among her favorite memories: “Reading and writing on the porch, which looked over the field of tadpole pots, early in the morning before everyone else woke up.”

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Hesse’s protagonist, Siddhartha, spends much of the book next to water as well, crossing a river several times in both me-taphorical and actual transitions from one life stage to the next. Early in the story Siddhartha realizes that wisdom cannot be gleaned solely by cutting himself off from the world and pursuing ideas in the abstract. Reading the passage where the physical world beckons to Siddhartha, it’s impossible not to think of a certain bearded field biologist, one who has never stopped enjoying the thrill of looking for salamanders in the woods at night: 

“Beautiful were the moon and the stars, beautiful was the stream and banks, the forest and the rocks . . . All of this had always existed, and he had not seen it; he had not been with it. Now he was with it, he was part of it. Light and shadow ran through his eyes, stars and moon ran through his heart.” 

Back in Portland on a sunny fall day, I sit in the Cerf amphitheatre and look east, toward the canyon. The colors of the red alder and bigleaf maple trees are turning and reflect in the water. Behind the pretty picture is a thriving ecosystem, one that’s been painstakingly restored since the late ’90s. For years Kaplan served as chair of the Canyon Committee, and his students still do research in the canyon. This year, Jenny Balmagia ’14 is studying the canyon’s macroinvertebrates, which includeseverything from worms and snails to crayfish and dragonflies.

  

Affection for Kaplan runs deep through the hearts of many former students. When I talk to Marshall on the phone, he’s just back from getting married on the Oregon coast. Of course Kaplan was there, and the two wound up laughing about Marshall’s first befuddling encounter with Siddhartha. 

Today Marshall is curator and collection manager at the Oregon State Arthropod Collection in Corvallis. He found his way back to bugs by way of Harvard and Cornell. He also found his way back to Hesse’s book, which he reread years after his Korea trip with Kaplan. 

“Now I get it,” says Marshall. “Your life changes don’t always seem like they all fit together, but you find your way—and you often become a different person as you transition from one stage to the next.”

Kaplan is still helping students find their way, especially down boggy, muddy trails in the dark. It’s a cold rainy day in November now, and we’re trading emails. He writes to warn me he’ll be tough to reach. He’ll be in the canyon with intro biology students all afternoon and then with upper-division students on Sauvie Island until 11 p.m that night. 

“We are looking for chorus frogs, rough-skinned newts, and long-toed salamanders; the first two species are already making their way to ponds to breed,” he writes. “Gotta do it!!!”

A protagonist, a journey, and a story about muddy boots and doing biology the old-fashioned way. Seems one that’s worth telling, and doing, more than once.